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KMID : 0361020000430070719
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Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 7 p.719 ~ p.723
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The Role of p38MAP Kinase Signal Transduction in the Induction of MUC 8 Gene Expression in Normal Human Nasal Epithelial Cells
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Yun Ju-Hyun
Lee Won-Jae
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Abstract
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Background and Objectives : In cystic fibrosis, chronic bronchitis, and asthma, the mucociliary mechanism
is impaired when mucin is produced excessively. The mRNA encoding MUC8 has been shown to be the
major up-regulated mucin under inflammatory condition and is likely to contribute to the airway mucus
plugging characteristic of these diseases. The aim of this study is to determine the intracellular signaling
pathway directly involved in the MUC8 regulation following inflammatory mediator treatments.
Materials and Method : Passage-2 normal human airway epithelial cells were used in all experiments.
Inflammatory signal-induced MAP kinase activity was measured by Western blot analysis using phosphospecific
anti-active MAP kinase antibodies. Inflammatory signal-induced MUC8 expression was measured in the
absence or absence or presence of SB203580 by the semi-quantative RT-PCR.
Results : Inflammatory stimuli such as LPS, TNF-α, and IL-1β activated the p38 MAP kinase and subsequently up-regulated the MUC8 expression, Interestingly, the TNF-α or IL-1β- inducibility of the MUC8 gene expression
was greatly enhanced by specific inhibition of the p38 MAP kinase by using SB 203580.
Conclusion : These results suggest that the intracellular p38 MAP kinase activity is a negative regulator for the MUC8 up-regulation in human nasal epithelial cells following inflammatory stimuli.
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KEYWORD
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Airway epithelial cells, p38MAP kinase, MUC8, Signal transduction
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